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Promega promega fugene 6
Promega Fugene 6, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/promega+fugene+6/fugene+hd/bio_rxiv__2025__07__11__659973-372-16-16
Average 90 stars, based on 1 article reviews
promega fugene 6 - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Assessing the Role of Post-Translational Modifications of Mitochondrial RNA Polymerase
Article Snippet: Plasmids were transfected using Promega FuGENE TM 6 (Fitchburg, WI, USA) following the manufacturer’s protocol.

Plasmid Preparation:

Article Title: Context-dependent regulatory variants in Alzheimer’s disease
Article Snippet: .. For each dish, 18 μg of the MPRA plasmid library was transfected using 54 μL of Promega FuGene 6. ..

Article Title: Stable Isotope-Assisted Untargeted Metabolomics Identifies ALDH1A1-Driven Erythronate Accumulation in Lung Cancer Cells
Article Snippet: .. Restriction site-based cloning was used to incorporate ALDH1A1 cDNA into the pBABE-puro plasmid, and the resulting plasmid was verified through sequencing. pBABE-puro-ALDH1A1 (or negative control empty pBABE-puro) was cotransfected into HEK-293T cells with pUMVC and pCMV-VSV-G plasmids using Promega FuGENE 6 (following manufacturer’s instructions) to produce retroviral particles. .. Spent 293T medium was harvested after 36 h, filtered through a 0.45 μm-pore membrane, and added with 8 μg/mL polybrene (Millipore, Burlington, MA, USA) to MCF-10A cells.

Article Title: Stable Isotope-Assisted Untargeted Metabolomics Identifies ALDH1A1-Driven Erythronate Accumulation in Lung Cancer Cells.
Article Snippet: .. Restriction site-based cloning was used to incorporate ALDH1A1 cDNA into the pBABEpuro plasmid, and the resulting plasmid was verified through sequencing. pBABE-puroALDH1A1 (or negative control empty pBABE-puro) was cotransfected into HEK-293T cells with pUMVC and pCMV-VSV-G plasmids using Promega FuGENE 6 (following manufacturer’s instructions) to produce retroviral particles. .. Spent 293T medium was harvested after 36 h, filtered through a 0.45 μm-pore membrane, and added with 8 μg/mL polybrene (Millipore, Burlington, MA, USA) to MCF-10A cells.

Transfection:

Article Title: Context-dependent regulatory variants in Alzheimer’s disease
Article Snippet: .. For each dish, 18 μg of the MPRA plasmid library was transfected using 54 μL of Promega FuGene 6. ..

Cloning:

Article Title: Stable Isotope-Assisted Untargeted Metabolomics Identifies ALDH1A1-Driven Erythronate Accumulation in Lung Cancer Cells
Article Snippet: .. Restriction site-based cloning was used to incorporate ALDH1A1 cDNA into the pBABE-puro plasmid, and the resulting plasmid was verified through sequencing. pBABE-puro-ALDH1A1 (or negative control empty pBABE-puro) was cotransfected into HEK-293T cells with pUMVC and pCMV-VSV-G plasmids using Promega FuGENE 6 (following manufacturer’s instructions) to produce retroviral particles. .. Spent 293T medium was harvested after 36 h, filtered through a 0.45 μm-pore membrane, and added with 8 μg/mL polybrene (Millipore, Burlington, MA, USA) to MCF-10A cells.

Article Title: Stable Isotope-Assisted Untargeted Metabolomics Identifies ALDH1A1-Driven Erythronate Accumulation in Lung Cancer Cells.
Article Snippet: .. Restriction site-based cloning was used to incorporate ALDH1A1 cDNA into the pBABEpuro plasmid, and the resulting plasmid was verified through sequencing. pBABE-puroALDH1A1 (or negative control empty pBABE-puro) was cotransfected into HEK-293T cells with pUMVC and pCMV-VSV-G plasmids using Promega FuGENE 6 (following manufacturer’s instructions) to produce retroviral particles. .. Spent 293T medium was harvested after 36 h, filtered through a 0.45 μm-pore membrane, and added with 8 μg/mL polybrene (Millipore, Burlington, MA, USA) to MCF-10A cells.

Sequencing:

Article Title: Stable Isotope-Assisted Untargeted Metabolomics Identifies ALDH1A1-Driven Erythronate Accumulation in Lung Cancer Cells
Article Snippet: .. Restriction site-based cloning was used to incorporate ALDH1A1 cDNA into the pBABE-puro plasmid, and the resulting plasmid was verified through sequencing. pBABE-puro-ALDH1A1 (or negative control empty pBABE-puro) was cotransfected into HEK-293T cells with pUMVC and pCMV-VSV-G plasmids using Promega FuGENE 6 (following manufacturer’s instructions) to produce retroviral particles. .. Spent 293T medium was harvested after 36 h, filtered through a 0.45 μm-pore membrane, and added with 8 μg/mL polybrene (Millipore, Burlington, MA, USA) to MCF-10A cells.

Article Title: Stable Isotope-Assisted Untargeted Metabolomics Identifies ALDH1A1-Driven Erythronate Accumulation in Lung Cancer Cells.
Article Snippet: .. Restriction site-based cloning was used to incorporate ALDH1A1 cDNA into the pBABEpuro plasmid, and the resulting plasmid was verified through sequencing. pBABE-puroALDH1A1 (or negative control empty pBABE-puro) was cotransfected into HEK-293T cells with pUMVC and pCMV-VSV-G plasmids using Promega FuGENE 6 (following manufacturer’s instructions) to produce retroviral particles. .. Spent 293T medium was harvested after 36 h, filtered through a 0.45 μm-pore membrane, and added with 8 μg/mL polybrene (Millipore, Burlington, MA, USA) to MCF-10A cells.

Negative Control:

Article Title: Stable Isotope-Assisted Untargeted Metabolomics Identifies ALDH1A1-Driven Erythronate Accumulation in Lung Cancer Cells
Article Snippet: .. Restriction site-based cloning was used to incorporate ALDH1A1 cDNA into the pBABE-puro plasmid, and the resulting plasmid was verified through sequencing. pBABE-puro-ALDH1A1 (or negative control empty pBABE-puro) was cotransfected into HEK-293T cells with pUMVC and pCMV-VSV-G plasmids using Promega FuGENE 6 (following manufacturer’s instructions) to produce retroviral particles. .. Spent 293T medium was harvested after 36 h, filtered through a 0.45 μm-pore membrane, and added with 8 μg/mL polybrene (Millipore, Burlington, MA, USA) to MCF-10A cells.

Article Title: Stable Isotope-Assisted Untargeted Metabolomics Identifies ALDH1A1-Driven Erythronate Accumulation in Lung Cancer Cells.
Article Snippet: .. Restriction site-based cloning was used to incorporate ALDH1A1 cDNA into the pBABEpuro plasmid, and the resulting plasmid was verified through sequencing. pBABE-puroALDH1A1 (or negative control empty pBABE-puro) was cotransfected into HEK-293T cells with pUMVC and pCMV-VSV-G plasmids using Promega FuGENE 6 (following manufacturer’s instructions) to produce retroviral particles. .. Spent 293T medium was harvested after 36 h, filtered through a 0.45 μm-pore membrane, and added with 8 μg/mL polybrene (Millipore, Burlington, MA, USA) to MCF-10A cells.

Retroviral:

Article Title: Stable Isotope-Assisted Untargeted Metabolomics Identifies ALDH1A1-Driven Erythronate Accumulation in Lung Cancer Cells
Article Snippet: .. Restriction site-based cloning was used to incorporate ALDH1A1 cDNA into the pBABE-puro plasmid, and the resulting plasmid was verified through sequencing. pBABE-puro-ALDH1A1 (or negative control empty pBABE-puro) was cotransfected into HEK-293T cells with pUMVC and pCMV-VSV-G plasmids using Promega FuGENE 6 (following manufacturer’s instructions) to produce retroviral particles. .. Spent 293T medium was harvested after 36 h, filtered through a 0.45 μm-pore membrane, and added with 8 μg/mL polybrene (Millipore, Burlington, MA, USA) to MCF-10A cells.

Article Title: Stable Isotope-Assisted Untargeted Metabolomics Identifies ALDH1A1-Driven Erythronate Accumulation in Lung Cancer Cells.
Article Snippet: .. Restriction site-based cloning was used to incorporate ALDH1A1 cDNA into the pBABEpuro plasmid, and the resulting plasmid was verified through sequencing. pBABE-puroALDH1A1 (or negative control empty pBABE-puro) was cotransfected into HEK-293T cells with pUMVC and pCMV-VSV-G plasmids using Promega FuGENE 6 (following manufacturer’s instructions) to produce retroviral particles. .. Spent 293T medium was harvested after 36 h, filtered through a 0.45 μm-pore membrane, and added with 8 μg/mL polybrene (Millipore, Burlington, MA, USA) to MCF-10A cells.



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